A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts

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Hayes , A , Nguyen , D , Andersson , M , Anton , A , Bailly , J-L , Beard , S , Benschop , K S M , Berginc , N , Blomqvist , S , Cunningham , E , Davis , D , Dembinski , J L , Diedrich , S , Dudman , S G , Dyrdak , R , Eltringham , G J A , Gonzales-Goggia , S , Gunson , R , Howson-Wells , H C , Jääskeläinen , A J , Lopez-Labrador , F X , Maier , M , Majumdar , M , Midgley , S , Mirand , A , Morley , U , Nordbo , S A , Oikarinen , S , Osman , H , Papa , A , Pellegrinelli , L , Piralla , A , Rabella , N , Richter , J , Smith , M , Strand , A S , Templeton , K , Vipond , B , Vuorinen , T , Williams , C , Wollants , E , Zakikhany , K , Fischer , T K , Harvala , H & Simmonds , P 2020 , ' A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts ' , Journal of Medical Virology , vol. 92 , no. 8 , pp. 1065-1074 . https://doi.org/10.1002/jmv.25659

Title: A European multicentre evaluation of detection and typing methods for human enteroviruses and parechoviruses using RNA transcripts
Author: Hayes, A.; Nguyen, D.; Andersson, M.; Anton, A.; Bailly, J-L; Beard, S.; Benschop, K. S. M.; Berginc, N.; Blomqvist, S.; Cunningham, E.; Davis, D.; Dembinski, J. L.; Diedrich, S.; Dudman, S. G.; Dyrdak, R.; Eltringham, G. J. A.; Gonzales-Goggia, S.; Gunson, R.; Howson-Wells, H. C.; Jääskeläinen, A. J.; Lopez-Labrador, F. X.; Maier, M.; Majumdar, M.; Midgley, S.; Mirand, A.; Morley, U.; Nordbo, S. A.; Oikarinen, S.; Osman, H.; Papa, A.; Pellegrinelli, L.; Piralla, A.; Rabella, N.; Richter, J.; Smith, M.; Strand, A. Söderlund; Templeton, K.; Vipond, B.; Vuorinen, T.; Williams, C.; Wollants, E.; Zakikhany, K.; Fischer, T. K.; Harvala, H.; Simmonds, P.
Other contributor: University of Helsinki, Medicum




Date: 2020-08
Language: eng
Number of pages: 10
Belongs to series: Journal of Medical Virology
ISSN: 0146-6615
DOI: https://doi.org/10.1002/jmv.25659
URI: http://hdl.handle.net/10138/325219
Abstract: Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) and human parechovirus (HPeV) infections. Its effectiveness depends critically on using the appropriate sample types and high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis and sepsis clinical presentation can be extremely low. This study evaluated the sensitivity and specificity of currently used commercial and in-house diagnostic and typing assays. Accurately quantified RNA transcript controls were distributed to 27 diagnostic and 12 reference laboratories in 17 European countries for blinded testing. Transcripts represented the four human EV species (EV-A71, echovirus 30, coxsackie A virus 21, and EV-D68), HPeV3, and specificity controls. Reported results from 48 in-house and 15 commercial assays showed 98% detection frequencies of high copy (1000 RNA copies/5 mu L) transcripts. In-house assays showed significantly greater detection frequencies of the low copy (10 copies/5 mu L) EV and HPeV transcripts (81% and 86%, respectively) compared with commercial assays (56%, 50%; P = 7 x 10(-5)). EV-specific PCRs showed low cross-reactivity with human rhinovirus C (3 of 42 tests) and infrequent positivity in the negative control (2 of 63 tests). Most or all high copy EV and HPeV controls were successfully typed (88%, 100%) by reference laboratories, but showed reduced effectiveness for low copy controls (41%, 67%). Stabilized RNA transcripts provide an effective, logistically simple and inexpensive reagent for evaluation of diagnostic assay performance. The study provides reassurance of the performance of the many in-house assay formats used across Europe. However, it identified often substantially reduced sensitivities of commercial assays often used as point-of-care tests.
Subject: enterovirus
enterovirus A71
parechovirus
PCR
RNA transcripts
CEREBROSPINAL-FLUID
CLINICAL-SAMPLES
INFECTIONS
EPIDEMIOLOGY
ASSOCIATION
RHINOVIRUS
DIVERSITY
CHILDREN
PANEL
11832 Microbiology and virology
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