Next generation organoids for biomedical research and applications

Näytä kaikki kuvailutiedot



Pysyväisosoite

http://hdl.handle.net/10138/327408

Lähdeviite

Lou , Y-R & Leung , A W 2018 , ' Next generation organoids for biomedical research and applications ' , Biotechnology Advances , vol. 36 , no. 1 , pp. 132-149 . https://doi.org/10.1016/j.biotechadv.2017.10.005

Julkaisun nimi: Next generation organoids for biomedical research and applications
Tekijä: Lou, Yan-Ru; Leung, Alan W.
Tekijän organisaatio: Faculty of Pharmacy
Division of Pharmaceutical Biosciences
Drug Research Program
Tissue engineering for drug research
Päiväys: 2018
Kieli: eng
Sivumäärä: 18
Kuuluu julkaisusarjaan: Biotechnology Advances
ISSN: 0734-9750
DOI-tunniste: https://doi.org/10.1016/j.biotechadv.2017.10.005
URI: http://hdl.handle.net/10138/327408
Tiivistelmä: Organoids are in vitro cultures of miniature fetal or adult organ-like structures. Their potentials for use in tissue and organ replacement, disease modeling, toxicology studies, and drug discovery are tremendous. Currently, major challenges facing human organoid technology include (i) improving the range of cellular heterogeneity for a particular organoid system, (ii) mimicking the native micro- and matrix-environment encountered by cells within organoids, and (iii) developing robust protocols for the in vitro maturation of organoids that remain mostly fetal-like in cultures. To tackle these challenges, we advocate the principle of reverse engineering that replicates the inner workings of in vivo systems with the goal of achieving functionality and maturation of the resulting organoid structures with the input of minimal intrinsic (cellular) and environmental (matrix and niche) constituents. Here, we present an overview of organoid technology development in several systems that employ cell materials derived from fetal and adult tissues and pluripotent stem cell cultures. We focus on key studies that exploit the self-organizing property of embryonic progenitors and the role of designer matrices and cell-free scaffolds in assisting organoid formation. We further explore the relationship between adult stem cells, niche factors, and other current developments that aim to enhance robust organoid maturation. From these works, we propose a standardized pipeline for the development of future protocols that would help generate more physiologically relevant human organoids for various biomedical applications.
Kuvaus: Corrigendum: Biotechnology Advances, Volume 37, Issue 3, May–June 2019, Page 505 DOI: 10.1016/j.biotechadv.2019.03.001 WOS: 000464301900010
Avainsanat: Organoids
Human
Reverse engineering
Self-organization
Designer matrix
Niche factors
Pluripotent stem cells
Drug screening
Disease modeling
Transplantation
PLURIPOTENT STEM-CELLS
IN-VITRO EXPANSION
DISSOCIATED EMBRYONIC CHICK
HUMAN CEREBRAL ORGANOIDS
NEURAL CREST INDUCTION
ADVERSE DRUG-REACTIONS
FUNCTIONAL HUMAN LIVER
HEPATOCYTE-LIKE CELLS
EX-VIVO EXPANSION
EXTRACELLULAR-MATRIX
317 Pharmacy
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by_nc_nd
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: acceptedVersion


Tiedostot

Latausmäärä yhteensä: Ladataan...

Tiedosto(t) Koko Formaatti Näytä
submitted_MS_text.pdf 1012.KB PDF Avaa tiedosto

Viite kuuluu kokoelmiin:

Näytä kaikki kuvailutiedot