A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens

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http://hdl.handle.net/10138/328169

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Azinheiro , S , Kant , K , Shahbazi , M-A , Garrido-Maestu , A , Prado , M & Dieguez , L 2020 , ' A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens ' , Food Control , vol. 114 , 107242 . https://doi.org/10.1016/j.foodcont.2020.107242

Title: A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens
Author: Azinheiro, Sarah; Kant, Krishna; Shahbazi, Mohammad-Ali; Garrido-Maestu, Alejandro; Prado, Marta; Dieguez, Lorena
Contributor organization: Division of Pharmaceutical Chemistry and Technology
Nanomedicines and Biomedical Engineering
Date: 2020-08
Language: eng
Number of pages: 7
Belongs to series: Food Control
ISSN: 0956-7135
DOI: https://doi.org/10.1016/j.foodcont.2020.107242
URI: http://hdl.handle.net/10138/328169
Abstract: Rapid and sensitive detection of foodborne pathogens in food industry is of high importance in day-to-day practice to ensure safe food. To address this issue, multiple foodborne pathogens are targeted for rapid identification based in DNA amplification. A 3D PDMS sponge was fabricated using salt crystals as scarifying mold and functionalized with a ligand, apolipoprotein-H (ApoH), to test bacterial capturing for both Gram positive (L. monocytogenes) and negative bacteria (Salmonella spp.), in a microfluidic device. Pure culture of both pathogens in a range of ∼10–105 CFU/mL were tested and the application of the developed automated pre-concentration protocol in real samples was verified using spiked surface samples after swab sampling. Bacterial DNA was extracted directly from the sponge and used for Real Time quantitative Polymerase Chain Reaction (qPCR) detection. The sponges did not show any significant resistance to sample flow and could easily be incorporated in a microfluidic device. A capture efficiency above 70% was observed for both targeted (Gram positive and Gram negative) pathogens and a Limit of Detection (LoD) in the range of 103 and 104 CFU/mL was obtained for Salmonella spp. and L. monocytogenes, respectively. Using this approached, we are able to perform multiplexed (Gram positive and Gram negative) capturing and reduce the enrichment time compared to the gold standard plate culture (over 1-day) method. The use of a 3D sponge for direct capturing of multiplexed pathogen on microfluidic device, followed by qPCR detection is an efficient and versatile method to stratify the presence of bacteria. This approach and methodology has potential to be integrated in full automatized device and used as point of need (PoN) system for foodborne pathogen stratification in food packaging/production industries.
Subject: 3D sponge
Foodborne pathogen
LISTERIA-MONOCYTOGENES
Microfluidic device
Multiplexed detection
PCR
SALMONELLA SPP.
SEPARATION
qPCR
11832 Microbiology and virology
416 Food Science
116 Chemical sciences
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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