A novel terpene synthase controls differences in anti-aphrodisiac pheromone production between closely related Heliconius butterflies

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http://hdl.handle.net/10138/328451

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Darragh , K , Orteu , A , Black , D , Byers , K J R P , Szczerbowski , D , Warren , I A , Rastas , P , Pinharanda , A , Davey , J W , Fernanda Garza , S , Abondano Almeida , D , Merrill , R M , McMillan , W O , Schulz , S & Jiggins , C D 2021 , ' A novel terpene synthase controls differences in anti-aphrodisiac pheromone production between closely related Heliconius butterflies ' , PLoS Biology , vol. 19 , no. 1 , 3001022 . https://doi.org/10.1371/journal.pbio.3001022

Title: A novel terpene synthase controls differences in anti-aphrodisiac pheromone production between closely related Heliconius butterflies
Author: Darragh, Kathy; Orteu, Anna; Black, Daniella; Byers, Kelsey J. R. P.; Szczerbowski, Daiane; Warren, Ian A.; Rastas, Pasi; Pinharanda, Ana; Davey, John W.; Fernanda Garza, Sylvia; Abondano Almeida, Diana; Merrill, Richard M.; McMillan, W. Owen; Schulz, Stefan; Jiggins, Chris D.
Contributor: University of Helsinki, Institute of Biotechnology
Date: 2021-01
Language: eng
Number of pages: 30
Belongs to series: PLoS Biology
ISSN: 1544-9173
URI: http://hdl.handle.net/10138/328451
Abstract: Plants and insects often use the same compounds for chemical communication, but not much is known about the genetics of convergent evolution of chemical signals. The terpene (E)-beta-ocimene is a common component of floral scent and is also used by the butterfly Heliconius melpomene as an anti-aphrodisiac pheromone. While the biosynthesis of terpenes has been described in plants and microorganisms, few terpene synthases (TPSs) have been identified in insects. Here, we study the recent divergence of 2 species, H. melpomene and Heliconius cydno, which differ in the presence of (E)-beta-ocimene; combining linkage mapping, gene expression, and functional analyses, we identify 2 novel TPSs. Furthermore, we demonstrate that one, HmelOS, is able to synthesise (E)-beta-ocimene in vitro. We find no evidence for TPS activity in HcydOS (HmelOS ortholog of H. cydno), suggesting that the loss of (E)-beta-ocimene in this species is the result of coding, not regulatory, differences. The TPS enzymes we discovered are unrelated to previously described plant and insect TPSs, demonstrating that chemical convergence has independent evolutionary origins.
Subject: RNA-SEQ EXPERIMENTS
DIPHOSPHATE SYNTHASE
EXPRESSION ANALYSIS
JUVENILE-HORMONE
MOLECULAR-BIOLOGY
FLORAL SCENTS
OPEN SOFTWARE
EVOLUTION
GENES
SELECTION
1182 Biochemistry, cell and molecular biology
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