An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners

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Räisänen , I T , Lähteenmäki , H , Gupta , S , Grigoriadis , A , Sahni , V , Suojanen , J , Seppänen , H , Tervahartiala , T , Sakellari , D & Sorsa , T 2021 , ' An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners ' , Diagnostics , vol. 11 , no. 4 , 711 . https://doi.org/10.3390/diagnostics11040711

Title: An aMMP-8 Point-of-Care and Questionnaire Based Real-Time Diagnostic Toolkit for Medical Practitioners
Author: Räisänen, Ismo T.; Lähteenmäki, Hanna; Gupta, Shipra; Grigoriadis, Andreas; Sahni, Vaibhav; Suojanen, Juho; Seppänen, Hanna; Tervahartiala, Taina; Sakellari, Dimitra; Sorsa, Timo
Other contributor: University of Helsinki, Doctoral Programme in Oral Sciences
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Plastiikkakirurgian yksikkö
University of Helsinki, CAN-PRO - Translational Cancer Medicine Program
University of Helsinki, Clinicum
University of Helsinki, HUS Head and Neck Center











Date: 2021-04
Language: eng
Number of pages: 8
Belongs to series: Diagnostics
ISSN: 2075-4418
DOI: https://doi.org/10.3390/diagnostics11040711
URI: http://hdl.handle.net/10138/330976
Abstract: The aim of this cross-sectional study is to propose an efficient strategy based on biomarkers adjunct with an interview/questionnaire covering risk factors for periodontitis for the identification of undiagnosed periodontitis by medical professionals. Active matrix metalloproteinase (aMMP)-8 levels in mouthrinse were analyzed by a point-of-care (PoC)/chairside lateral-flow immunotest, and salivary total MMP-8, total MMP-9 and calprotectin levels were analyzed by enzyme-linked immunosorbent assays (ELISAs) and active MMP-9 by gelatin zymography for 149 Greek patients. Patients underwent a full-mouth oral health examination for diagnosis according to the 2018 classification system of periodontal diseases. In addition, patient characteristics (risk factors: age, gender, education level, smoking and body mass index) were recorded. Receiver operating curve (ROC) analysis indicated better diagnostic precision to identify undiagnosed periodontitis for oral fluid biomarkers in adjunct with an interview/questionnaire compared with a plain questionnaire (i.e., risk factors): aMMP-8 AUC (95% confidence interval) = 0.834 (0.761-0.906), total MMP-8 = 0.800 (0.722-0.878), active MMP-9 = 0.787 (0.704-0.870), total MMP-9 = 0.773 (0.687-0.858) and calprotectin = 0.773 (0.687-0.858) vs. questionnaire = 0.764 (0.676-0.851). The findings of this study suggest that oral fluid biomarker analysis, such as a rapid aMMP-8 PoC immunotest, could be used as an adjunct to an interview/questionnaire to improve the precision of timely identification of asymptomatic, undiagnosed periodontitis patients by medical professionals. This strategy appears to be viable for referring patients to a dentist for diagnosis and treatment need assessment.
Subject: periodontitis
diagnostics
point-of-care systems
biomarkers
matrix metalloproteinase 8
preventive medicine
oral health
316 Nursing
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