A physiologically validated rat model of term birth asphyxia with seizure generation after, not during, brain hypoxia

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Ala-Kurikka , T , Pospelov , A , Summanen , M , Alafuzoff , A , Kurki , S , Voipio , J & Kaila , K 2021 , ' A physiologically validated rat model of term birth asphyxia with seizure generation after, not during, brain hypoxia ' , Epilepsia , vol. 62 , no. 4 , pp. 908-919 . https://doi.org/10.1111/epi.16790

Title: A physiologically validated rat model of term birth asphyxia with seizure generation after, not during, brain hypoxia
Author: Ala-Kurikka, Tommi; Pospelov, Alexey; Summanen, Milla; Alafuzoff, Aleksander; Kurki, Samu; Voipio, Juha; Kaila, Kai
Other contributor: University of Helsinki, Laboratory of Neurobiology
University of Helsinki, Laboratory of Neurobiology
University of Helsinki, Laboratory of Neurobiology
University of Helsinki, Laboratory of Neurobiology
University of Helsinki, Faculty of Biological and Environmental Sciences
University of Helsinki, Juha Voipio / Principal Investigator
University of Helsinki, Kai Kaila / Principal Investigator







Date: 2021-04
Language: eng
Number of pages: 12
Belongs to series: Epilepsia
ISSN: 0013-9580
DOI: https://doi.org/10.1111/epi.16790
URI: http://hdl.handle.net/10138/331433
Abstract: Objective Birth asphyxia (BA) is often associated with seizures that may exacerbate the ensuing hypoxic-ischemic encephalopathy. In rodent models of BA, exposure to hypoxia is used to evoke seizures, that commence already during the insult. This is in stark contrast to clinical BA, in which seizures are typically seen upon recovery. Here, we introduce a term-equivalent rat model of BA, in which seizures are triggered after exposure to asphyxia. Methods Postnatal day 11-12 male rat pups were exposed to steady asphyxia (15 min; air containing 5% O-2 + 20% CO2) or to intermittent asphyxia (30 min; three 5 + 5-min cycles of 9% and 5% O-2 at 20% CO2). Cortical activity and electrographic seizures were recorded in freely behaving animals. Simultaneous electrode measurements of intracortical pH, Po-2, and local field potentials (LFPs) were made under urethane anesthesia. Results Both protocols decreased blood pH to Significance The rate of brain pH recovery has a strong influence on post-asphyxia seizure propensity. The recurring hypoxic episodes during intermittent asphyxia promote neuronal excitability, which leads to seizures only after the suppressing effect of the hypercapnic acidosis is relieved. The present rodent model of BA is to our best knowledge the first one in which, consistent with clinical BA, behavioral and electrographic seizures are triggered after and not during the BA-mimicking insult.
Subject: chemoreflex
hypercapnia
hypoxia
neonatal seizures
neuroprotection
perinatal asphyxia
INTRACELLULAR PH
INJURY
ELECTROENCEPHALOGRAM
POTENTIATION
ALKALOSIS
EVOLUTION
RESPONSES
EVENTS
BURDEN
RISK
3112 Neurosciences
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