Targeting the Peroxisomes for Synthesizing Polyketides by Type-III PKSs Enzymes in Tobacco and Petunia

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http://urn.fi/URN:NBN:fi:hulib-202106183165
Title: Targeting the Peroxisomes for Synthesizing Polyketides by Type-III PKSs Enzymes in Tobacco and Petunia
Author: Rashid, Mamunur
Other contributor: Helsingin yliopisto, Maatalous-metsätieteellinen tiedekunta
University of Helsinki, Faculty of Agriculture and Forestry
Helsingfors universitet, Agrikultur- och forstvetenskapliga fakulteten
Publisher: Helsingin yliopisto
Date: 2021
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-202106183165
http://hdl.handle.net/10138/331548
Thesis level: master's thesis
Degree program: Erasmus-Mundus Master Program in Plant Breeding (emPlant)
Erasmus-Mundus Master Program in Plant Breeding (emPlant)
Erasmus-Mundus Master Program in Plant Breeding (emPlant)
Specialisation: Kasvintuotantotieteet
Plant Production Sciences
Växtproduktionsvetenskaper
Abstract: Secondary metabolites are bioactive compounds that help the plant to adapt in different adverse environmental conditions but are not an essential part of plant developmental processes and also secondary metabolites have pharmaceutical value because of their antioxidant, anticancer, antibacterial and antifungal properties. Type-III polyketide synthases (PKSs) are a group of polyketide synthases that produce secondary metabolites with diverse biological activities in plants. The main objective was to localize the PKSs G2PS1, G2PS2, FvCHS2-1, HlVPS into the peroxisomes for synthesizing secondary metabolites in plants. The experiment was performed by amplifying the genes with specific Px-targeting signal. Then the genes cloned into destination vector pEAQ-HT-DEST1. The plasmid constructs were transformed to Agrobacterium tumefaciens and agro-infiltrated to Nicotiana benthamiana and Petunia hybrida leaf tissue. Western blotting results revealed that all proteins were expressed in infiltrated leaves of both tobacco and petunia but HPLC chromatograms showed that only the protein FvCHS2-1 produced novel peaks for metabolites in tobacco.
Subject: Secondary metabolites
Type-III PKSs
Peroxisomes
Tobacco
Petunia


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