Active mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitis

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Hernández , M , Baeza , M , Räisänen , I T , Contreras , J , Tervahartiala , T , Chaparro , A , Sorsa , T & Hernández-Ríos , P 2021 , ' Active mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitis ' , Diagnostics , vol. 11 , no. 8 , 1503 . https://doi.org/10.3390/diagnostics11081503

Title: Active mmp-8 quantitative test as an adjunctive tool for early diagnosis of periodontitis
Author: Hernández, Marcela; Baeza, Mauricio; Räisänen, Ismo T.; Contreras, Johanna; Tervahartiala, Taina; Chaparro, Alejandra; Sorsa, Timo; Hernández-Ríos, Patricia
Contributor organization: Department of Oral and Maxillofacial Diseases
HUS Head and Neck Center
Clinicum
Suu- ja leukakirurgian yksikkö
Date: 2021-08
Language: eng
Number of pages: 11
Belongs to series: Diagnostics
ISSN: 2075-4418
DOI: https://doi.org/10.3390/diagnostics11081503
URI: http://hdl.handle.net/10138/335315
Abstract: Periodontitis is a host-mediated bacterial disease that affects the tooth attachment apparatus. Metalloproteinase-8 (MMP-8), a validated biomarker, could aid in clinical diagnosis. This study aimed to evaluate the diagnostic performance of active (a) MMP-8 immunotest versus total (t) MMP-8 ELISA for quantitative real-time diagnosis and assessment of periodontitis severity at the site level. Gingival crevicular fluid (GCF) was sampled from 30 healthy, 42 mild, and 59 severe periodontitis sites from thirty-one volunteers. MMP-8 concentrations were determined by time-resolved immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed using the STATA package. Both active and total MMP-8-based methods discriminated among sites according to periodontal diagnosis and severity, with a positive correlation between the two tests (p < 0.001). (a) MMP-8 models showed the best performance in receiver operating characteristic (ROC) curves to discriminate between healthy and periodontitis sites (area under the curve [AUC] = 0.89), while (t) MMP-8 demonstrated a high diagnostic precision in the detection of mild from severe periodontitis sites (AUC ≥ 0.80). The use of (a) MMP-8 and (t) MMP-8 could represent a useful adjunctive tool for periodontitis diagnosis and severity. These results support the applicability of new point-of-care methods in the monitoring of high-risk periodontal patients.
Description: Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subject: Biomarkers
Diagnosis
Gingival crevicular fluid
Matrix metalloproteinase-8
Periodontal disease
Periodontitis
NEUTROPHIL COLLAGENASE ACTIVITY
matrix metalloproteinase-8
biomarkers
MARKERS
diagnosis
GINGIVAL CREVICULAR FLUID
CLASSIFICATION
MATRIX METALLOPROTEINASE-8
periodontal disease
BIOMARKERS
TISSUE DESTRUCTION
periodontitis
gingival crevicular fluid
DEGRADATION
HEALTH
PROGRESSION
313 Dentistry
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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