ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response

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Pascual , J , Rahikainen , M , Angeleri , M , Alegre , S , Gossens , R , Shapiguzov , A , Heinonen , A , Trotta , A , Durian , G , Winter , Z , Sinkkonen , J , Kangasjarvi , J , Whelan , J & Kangasjärvi , S 2021 , ' ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response ' , Plant Physiology , vol. 186 , no. 4 , pp. 1859-1877 . https://doi.org/10.1093/plphys/kiab225

Title: ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response
Author: Pascual, Jesus; Rahikainen, Moona; Angeleri, Martina; Alegre, Sara; Gossens, Richard; Shapiguzov, Alexey; Heinonen, Arttu; Trotta, Andrea; Durian, Guido; Winter, Zsofia; Sinkkonen, Jari; Kangasjarvi, Jaakko; Whelan, James; Kangasjärvi, Saijaliisa
Other contributor: University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Organismal and Evolutionary Biology Research Programme
University of Helsinki, Faculty of Biological and Environmental Sciences
University of Helsinki, Viikki Plant Science Centre (ViPS)






Date: 2021-08
Language: eng
Number of pages: 19
Belongs to series: Plant Physiology
ISSN: 0032-0889
DOI: https://doi.org/10.1093/plphys/kiab225
URI: http://hdl.handle.net/10138/335630
Abstract: Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Posttranslational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B'gamma, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3(S91D)-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against ultraviolet B (UV-B) or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.
Subject: ALTERNATIVE OXIDASE
OXIDATIVE STRESS
GENE-EXPRESSION
PLANT
ARABIDOPSIS
IDENTIFICATION
METABOLISM
PP2A-B'GAMMA
RESISTANCE
INDUCTION
11831 Plant biology
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