A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics

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Jalkanen , P , Pasternack , A , Maljanen , S , Melen , K , Kolehmainen , P , Huttunen , M , Lundberg , R , Tripathi , L , Khan , H , Ritvos , M A , Naves , R , Haveri , A , Österlund , P , Kuivanen , S , Jääskeläinen , A J , Kurkela , S , Lappalainen , M , Rantasärkkä , K , Vuorinen , T , Hytönen , J , Waris , M , Tauriainen , S , Ritvos , O , Kakkola , L & Julkunen , I 2021 , ' A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics ' , Journal of Infectious Diseases , vol. 224 , no. 2 , pp. 218-228 . https://doi.org/10.1093/infdis/jiab222

Title: A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics
Author: Jalkanen, Pinja; Pasternack, Arja; Maljanen, Sari; Melen, Krister; Kolehmainen, Pekka; Huttunen, Moona; Lundberg, Rickard; Tripathi, Lav; Khan, Hira; Ritvos, Mikael A.; Naves, Rauno; Haveri, Anu; Österlund, Pamela; Kuivanen, Suvi; Jääskeläinen, Anne J.; Kurkela, Satu; Lappalainen, Maija; Rantasärkkä, Kaisa; Vuorinen, Tytti; Hytönen, Jukka; Waris, Matti; Tauriainen, Sisko; Ritvos, Olli; Kakkola, Laura; Julkunen, Ilkka
Contributor organization: Medicum
Department of Physiology
Faculty of Medicine
Viral Zoonosis Research Unit
Department of Virology
Staff Services
Olli Pekka Vapalahti / Principal Investigator
Growth factor physiology
Date: 2021-07-15
Language: eng
Number of pages: 11
Belongs to series: Journal of Infectious Diseases
ISSN: 0022-1899
DOI: https://doi.org/10.1093/infdis/jiab222
URI: http://hdl.handle.net/10138/335678
Abstract: Background. Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates. Methods. We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), Si and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results. Results. The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. Si and RBD-based EIA results had a strong correlation with microneutralization test results. Conclusions. The data indicate a combination of SARS-CoV-2 Si or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Subject: COVID-19
enzyme immunoassay
respiratory infection
coronavirus proteins
neutralizing antibodies
3111 Biomedicine
11832 Microbiology and virology
3121 General medicine, internal medicine and other clinical medicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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