A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics

Show full item record



Permalink

http://hdl.handle.net/10138/335678

Citation

Jalkanen , P , Pasternack , A , Maljanen , S , Melen , K , Kolehmainen , P , Huttunen , M , Lundberg , R , Tripathi , L , Khan , H , Ritvos , M A , Naves , R , Haveri , A , Österlund , P , Kuivanen , S , Jääskeläinen , A J , Kurkela , S , Lappalainen , M , Rantasärkkä , K , Vuorinen , T , Hytönen , J , Waris , M , Tauriainen , S , Ritvos , O , Kakkola , L & Julkunen , I 2021 , ' A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics ' , Journal of Infectious Diseases , vol. 224 , no. 2 , pp. 218-228 . https://doi.org/10.1093/infdis/jiab222

Title: A Combination of N and S Antigens With IgA and IgG Measurement Strengthens the Accuracy of SARS-CoV-2 Serodiagnostics
Author: Jalkanen, Pinja; Pasternack, Arja; Maljanen, Sari; Melen, Krister; Kolehmainen, Pekka; Huttunen, Moona; Lundberg, Rickard; Tripathi, Lav; Khan, Hira; Ritvos, Mikael A.; Naves, Rauno; Haveri, Anu; Österlund, Pamela; Kuivanen, Suvi; Jääskeläinen, Anne J.; Kurkela, Satu; Lappalainen, Maija; Rantasärkkä, Kaisa; Vuorinen, Tytti; Hytönen, Jukka; Waris, Matti; Tauriainen, Sisko; Ritvos, Olli; Kakkola, Laura; Julkunen, Ilkka
Contributor: University of Helsinki, Medicum
University of Helsinki, Department of Physiology
University of Helsinki, Faculty of Medicine
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Clinicum
University of Helsinki, Department of Physiology
Date: 2021-07-15
Language: eng
Number of pages: 11
Belongs to series: Journal of Infectious Diseases
ISSN: 0022-1899
URI: http://hdl.handle.net/10138/335678
Abstract: Background. Primary diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is based on detection of virus RNA in nasopharyngeal swab samples. In addition, analysis of humoral immunity against SARS-CoV-2 has an important role in viral diagnostics and seroprevalence estimates. Methods. We developed and optimized an enzyme immunoassays (EIA) using SARS-CoV-2 nucleoprotein (N), Si and receptor binding domain (RBD) of the viral spike protein, and N proteins from SARS, Middle East respiratory syndrome (MERS), and 4 low-pathogenic human CoVs. Neutralizing antibody activity was compared with SARS-CoV-2 IgG, IgA, and IgM EIA results. Results. The sensitivity of EIA for detecting immune response in COVID-19 patients (n = 101) was 77% in the acute phase and 100% in the convalescent phase of SARS-CoV-2 infection when N and RBD were used as antigens in IgG and IgA specific EIAs. SARS-CoV-2 infection significantly increased humoral immune responses against the 229E and NL63 N proteins. Si and RBD-based EIA results had a strong correlation with microneutralization test results. Conclusions. The data indicate a combination of SARS-CoV-2 Si or RBD and N proteins and analysis of IgG and IgA immunoglobulin classes in sera provide an excellent basis for specific and sensitive serological diagnostics of COVID-19.
Subject: COVID-19
SARS-CoV-2
enzyme immunoassay
serology
respiratory infection
antibodies
coronavirus proteins
neutralizing antibodies
HUMAN CORONAVIRUSES 229E
CROSS-REACTIVITY
ANTIBODIES
INFECTION
RESPONSES
3111 Biomedicine
11832 Microbiology and virology
3121 General medicine, internal medicine and other clinical medicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
jiab222.pdf 2.932Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record