Z-scores for comparative analyses of spermatogonial numbers throughout human development

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Funke , M , Yang , Y , Lahtinen , A , Benninghoven-Frey , K , Kliesch , S , Neuhaus , N , Stukenborg , J-B & Jahnukainen , K 2021 , ' Z-scores for comparative analyses of spermatogonial numbers throughout human development ' , Fertility and Sterility : official journal of the American Fertility Society , vol. 116 , no. 3 , pp. 713-720 . https://doi.org/10.1016/j.fertnstert.2021.04.019

Title: Z-scores for comparative analyses of spermatogonial numbers throughout human development
Author: Funke, Miriam; Yang, Yifan; Lahtinen, Atte; Benninghoven-Frey, Klara; Kliesch, Sabine; Neuhaus, Nina; Stukenborg, Jan-Bernd; Jahnukainen, Kirsi
Other contributor: University of Helsinki, ATG - Applied Tumor Genomics
University of Helsinki, Children's Hospital



Date: 2021-08
Language: eng
Number of pages: 8
Belongs to series: Fertility and Sterility : official journal of the American Fertility Society
ISSN: 0015-0282
DOI: https://doi.org/10.1016/j.fertnstert.2021.04.019
URI: http://hdl.handle.net/10138/335932
Abstract: Objective: To normalize age-dependent effects on standardized measures of spermatogonial quantity such as the number of spermatogonia per tubular cross-section (S/T) or fertility index. Design: Published quantitative histologic data on human spermatogonial numbers were used to create Z-scores for reference means and tested on archived testicular tissue samples. Setting: Retrospective cohort study. Patient(s): The sample cohort comprised testicular samples from 24 boys with cancer diagnosis and 10 with Klinefelter syndrome, as part of the fertility preservation programs NORDFERTIL and Androprotect, as well as archived histologic samples from 35 prepubertal boys with acute lymphoblastic leukemia and 20 testicular biobank samples. Intervention(s): None. Main Outcome Measure(s): Z-score values for S/T and fertility index on the basis of morphology and germ cell-specific markers (MAGEA4 and/or DDX4) were calculated, and the impact of cancer therapy exposure and genetic disorders on Z-score values was evaluated. Result(s): The Z-scores for S/T values in the nontreated samples (-2.08 +/- 2.20, n = 28) and samples treated with nonalkylating agents (-1.90 +/- 2.60, n = 25) were comparable within +/- 3 standard deviations of the reference mean value but differed significantly from samples exposed to alkylating agents (-12.14 +/- 9.20, n = 22) and from patients with Klinefelter syndrome (-11.56 +/- 4.89, n = 8). The Z-scores for S/T were correlated with increasing cumulative exposure to alkylating agents (r = -0.7020). Conclusion(s): The Z-score values for S/T allow for the quantification of genetic and cancer treatment-related effects on testicular tissue stored for fertility preservation, facilitating their use for patient counseling. (C) 2021 by American Society for Reproductive Medicine.
Subject: Klinefelter syndrome
male fertility preservation
spermatogonia
cancer
Z-score
HUMAN TESTIS
BOYS
CRYPTORCHID
HISTOLOGY
QUANTITY
ONSET
3123 Gynaecology and paediatrics
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